readIdatFiles(crlmm)
readIdatFiles()所属R语言包:crlmm
Reads Idat Files from Infinium II Illumina BeadChips
读取Infinium二Illumina的BeadChips IDAT文件
译者:生物统计家园网 机器人LoveR
描述----------Description----------
Reads intensity information for each bead type from
读取每个珠型的强度信息
用法----------Usage----------
readIdatFiles(sampleSheet=NULL, arrayNames=NULL, ids=NULL, path="",
arrayInfoColNames=list(barcode="SentrixBarcode_A",
position="SentrixPosition_A"),
highDensity=FALSE, sep="_",
fileExt=list(green="Grn.idat", red="Red.idat"),
saveDate=FALSE, verbose=FALSE)
参数----------Arguments----------
参数:sampleSheet
data.frame containing Illumina sample sheet information (for required columns, refer to BeadStudio Genotyping guide - Appendix A).
data.frame含有Illumina的样品表信息(所需的列,请参阅BeadStudio基因分型指南 - 附录A)。
参数:arrayNames
character vector containing names of arrays to be read in. If NULL, all arrays that can be found in the specified working directory will be read in.
包含数组的名字被读入。如果NULL,可以在指定的工作目录中找到的所有阵列将读入的字符向量
参数:ids
vector containing ids of probes to be read in. If NULL all probes found on the first array are read in.
向量的探针被读入,如果NULL上发现的第一个数组的所有探针都读入的ID
参数:path
character string specifying the location of files to be read by the function
字符串功能读取指定文件的位置
参数:arrayInfoColNames
(used when sampleSheet is specified) list containing elements 'barcode' which indicates column names in the sampleSheet which contains the arrayNumber/barcode number and 'position' which indicates the strip number. In older style sample sheets, this information is combined (usually in a column named 'SentrixPosition') and this should be specified as list(barcode=NULL, position="SentrixPosition")
(用时sampleSheet指定)包含元素的条码“表示sampleSheet其中包含的arrayNumber /条码号和”立场“表示条数列名的列表。在旧式的样品表,此信息相结合(通常在一列名为“SentrixPosition”),这应作为list(barcode=NULL, position="SentrixPosition")指定
参数:highDensity
logical (used when sampleSheet is specified). If TRUE, array extensions '\_A', '\_B' in sampleSheet are replaced with 'R01C01', 'R01C02' etc.
逻辑(当sampleSheet指定使用)。如果TRUE,阵列扩展\ _A,\ _B“在sampleSheet取代R01C01,R01C02”等。
参数:sep
character string specifying separator used in .idat file names.
字符串指定分隔符使用。IDAT文件名。
参数:fileExt
list containing elements 'Green' and 'Red' which specify the .idat file extension for the Cy3 and Cy5 channels.
列表,其中包含元素的绿色和红指定文件扩展名。IDAT Cy3和Cy5通道。
参数:saveDate
logical. Should the dates from each .idat be saved with sample information?
逻辑。应该从每个。IDAT日期与样品信息被保存?
参数:verbose
logical. Should processing information be displayed as data is read in?
逻辑。应处理的信息显示的数据被读取?
Details
详情----------Details----------
The summarised Cy3 (G) and Cy5 (R) intensities (on the orginal scale) are read in from the .idat files.
汇总Cy3标记(七)和Cy5(R)的强度(原价规模)。IDAT文件读取从。
Where available, a sampleSheet data.frame, in the same format as used by BeadStudio (columns 'Sample\_ID', 'SentrixBarcode\_A' and 'SentrixPosition\_A' are required) which keeps track of sample information can be specified.
如果可用,sampleSheet数据框在保持样品信息跟踪BeadStudio(列的范例\的_ID SentrixBarcode \ _A和SentrixPosition \ _A“要求的)使用相同的格式,可以被指定。
Thanks to Keith Baggerly who provided the code to read in the binary .idat files.
基思Baggerly谁提供的二进制代码来读取。IDAT文件。
值----------Value----------
NChannelSet with intensity data (R, G), and indicator for SNPs with 0 beads (zero) for each bead type.
R强度数据NChannelSet(,G),单核苷酸多态性指标与0珠(zero)为每个珠类型。
作者(S)----------Author(s)----------
Matt Ritchie
参考文献----------References----------
R/Bioconductor software for Illumina's Infinium whole-genome genotyping BeadChips. Bioinformatics. 2009 Oct 1;25(19):2621-3.
举例----------Examples----------
#RG = readIdatFiles()[RG = readIdatFiles()]
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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