metrics-methods(beadarray)
metrics-methods()所属R语言包：beadarray
Accessing metrics information in bead-level objects
在珠级别对象访问度量信息
译者：生物统计家园网 机器人LoveR
描述----------Description----------
The metrics, p95 and snr methods conveniently access metrics information that is stored in a beadLevelData object. These data are generated by the Illumina scanning software and can be an early indicator of array quality. These include the 95th (P95) and 5th (P05) quantiles of all pixel intensities on the image. A signal-to-noise ratio (SNR) can be calculated as the ratio of these two quantities. These metrics can be viewed in real-time as the arrays themselves are being scanned. By tracking these metrics over time, one can potentially halt problematic experiments before they even reach the analysis stage. Illumina recommend that the SNR ratio should be above 10, so these arrays are acceptable. However, the P95 and P05 values will fluctuate over time and are dependant upon the scanner setup. Including SNR values for arrays other than those currently being analysed will give a better indication of whether any outlier arrays exist.
metrics，p95和snr方法方便地访问度量信息存储在beadLevelData对象。这些数据生成由Illumina的扫描软件，可以是数组质量的早期指标。这些措施包括在图像上所有像素的强度95（P95）和第5（P05，P06）位数。一个信号噪声比（SNR），可以计算出这两个量的比例。这些指标可以作为自己正在扫描阵列实时观看。通过跟踪这些指标随着时间的推移，人们可能会停止有问题的实验之前，他们甚至达到了分析阶段。 Illumina的建议信噪比的比例应高于10，所以这些阵列是可以接受的。然而，P95和P05，P06值会随着时间的推移而波动，取决于扫描仪设置。包括信噪比值比目前正在分析这些阵列将提供一个更好的指示是否存在任何离群阵列。
作者（S）----------Author(s)----------
Mark Dunning
举例----------Examples----------
if(require(beadarrayExampleData)){
data(exampleBLData)
metrics(exampleBLData)
p95(exampleBLData, "Grn")
snr(exampleBLData, "Grn")
}
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注：
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发表于 2012-2-25 12:43:44
