normalize.qspline(affy)
normalize.qspline()所属R语言包:affy
Normalize arrays
标准化阵列
译者:生物统计家园网 机器人LoveR
描述----------Description----------
normalizes arrays in an AffyBatch each other or to a set of target intensities
在一个AffyBatch对方或一套目标强度标准化阵列
用法----------Usage----------
normalize.AffyBatch.qspline(abatch,type=c("together", "pmonly", "mmonly",
"separate"), ...)
normalize.qspline(x, target = NULL, samples = NULL,
fit.iters = 5, min.offset = 5,
spline.method = "natural", smooth = TRUE,
spar = 0, p.min = 0, p.max = 1.0,
incl.ends = TRUE, converge = FALSE,
verbose = TRUE, na.rm = FALSE)
参数----------Arguments----------
参数:x
a data.matrix of intensities
data.matrix强度
参数:abatch
an AffyBatch
AffyBatch
参数:target
numerical vector of intensity values to normalize to. (could be the name for one of the celfiles in 'abatch').
强度值的数值向量标准化。 (可能为一体的在abatch“celfiles的名称)。
参数:samples
numerical, the number of quantiles to be used for spline. if (0,1], then it is a sampling rate.
数值,样条位数的号码。如果(0,1],那么它是一个采样率。
参数:fit.iters
number of spline interpolations to average.
样条插值的平均数量。
参数:min.offset
minimum span between quantiles (rank difference) for the different fit iterations.
适合不同迭代位数(排名差异)之间的最小跨度。
参数:spline.method
specifies the type of spline to be used. Possible values are ""fmm"", ""natural"", and ""periodic"".
指定要使用花键的类型。可能的值是“FMM”,“自然”“,”定期“。
参数:smooth
logical, if "TRUE", smoothing splines are used on the quantiles.
逻辑,如果“TRUE”,平滑样条上使用的位数。
参数:spar
smoothing parameter for "splinefun", typically in (0,1].
平滑参数splinefun,通常在(0,1]。
参数:p.min
minimum percentile for the first quantile.
第一位数的最低百分。
参数:p.max
maximum percentile for the last quantile.
最后位数的最大百分。
参数:incl.ends
include the minimum and maximum values from the normalized and target arrays in the fit.
包括在适当的规范化和目标阵列的最大值和最小值。
参数:converge
(currently unimplemented)
(目前还没有实现)
参数:verbose
logical, if "TRUE" then normalization progress is reported.
逻辑,如果“TRUE”标准化的进展报告。
参数:na.rm
logical, if "TRUE" then handle NA values (by ignoring them).
逻辑,如果“TRUE”,然后处理不适用值(忽略)。
参数:type
a string specifying how the normalization should be applied. See details for more.
一个字符串,指定应如何标准化。看到更多的细节。
参数:...
optional parameters to be passed through.
可选参数,可以通过。
Details
详情----------Details----------
This normalization method uses the quantiles from each array and the target to fit a system of cubic splines to normalize the data. The target should be the mean (geometric) or median of each probe but could also be the name of a particular chip in the abatch object.
这标准化的方法是使用每个阵列和目标,以适应系统的三次样条标准化数据的位数。我们的目标应该是平均(几何)或中位数每个探针,但也可能是一个特定芯片在abatch对象的名称。
Parameters setting can be of much importance when using this method. The parameter fit.iter is used as a starting point to find a more appropriate value. Unfortunately the algorithm used do not converge in some cases. If this happens, the fit.iter value is used and a warning is thrown. Use of different settings for the parameter samples was reported to give good results. More specifically, for about 200 data points use samples = 0.33, for about 2000 data points use samples = 0.05, for about 10000 data points use samples = 0.02 (thanks to Paul Boutros).
使用此方法时,参数的设置,可以是非常重视。参数fit.iter作为出发点,以找到一个更合适的值。不幸的是,所使用的算法在某些情况下不收敛。如果发生这种情况,fit.iter值被使用,则抛出一个警告。使用不同的设置参数samples据报道,给予了良好的效果。更具体地说,使用约200个数据点samples = 0.33,约2000个数据点使用samples = 0.05,约10000个数据点使用samples = 0.02(感谢保罗·布特罗斯)。
The type argument should be one of "separate","pmonly","mmonly","together" which indicates whether to normalize only one probe type (PM,MM) or both together or separately.
type参数应该是一个"separate","pmonly","mmonly","together"这表明是否只有一个探针类型(PM,MM)或一起或单独标准化。
值----------Value----------
a normalized AffyBatch.
归一AffyBatch。
作者(S)----------Author(s)----------
Laurent and Workman C.
参考文献----------References----------
Laurent Gautier, Henrik Bjorn Nielsen, Hans-Henrik Saxild, Claus Nielsen, Soren Brunak, and Steen Knudsen. A new non-linear normal- ization method for reducing variability in dna microarray experiments. Genome Biology, accepted, 2002
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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