topGene(RankProd)
topGene()所属R语言包:RankProd
Output Significant Genes
输出的重要基因
译者:生物统计家园网 机器人LoveR
描述----------Description----------
Identify differentially expressed genes using
识别差异表达的基因使用
用法----------Usage----------
参数----------Arguments----------
参数:x
the value returned by the function RP, RP.advance or Rsum.advance
返回函数反相,RP.advance或Rsum.advance的价值
参数:cutoff
threshold in pfp used to select genes
在亲民党的阈值来选择基因
参数:method
If cutoff is provided, the method needs to be selected to identify genes."pfp" uses percentage of false prediction, which is the default setting. "pval" used p-value which is less stringent than pfp
如果提供截止,该方法需要选择,以确定基因。“亲民党”使用虚假的预测,这是默认设置的百分比。 “pval的”使用p值小于亲民党严格
参数:num.gene
number of candidate genes of interests, if cutoff is provided, this will be ignored
候选基因数目的利益,如果提供截止,这将被忽略
参数:logged
if "TRUE", data has bee logged, otherwise set it to "FALSE"
如果“真”,数据记录了蜜蜂,否则将其设置为“FALSE”
参数:logbase
base used when taking log, used to restore the fold change.The default value is 2, this will be ignored if logged=FALSE
碱基使用时,log,用于恢复的褶皱改变之默认值是2,这个记录将被忽略,如果为FALSE
参数:gene.names
if "NULL", no gene name will be attached to the output table
如果“空”,没有基因的名称将被连接到输出表
值----------Value----------
Two tables of identified genes with gene.index: index of gene in the original data set RP/Rsum: Computed rank product/sum for each gene FCclass1/class2): Expression Fold change of class 1/ class 2. pfp: estimated pfp for each gene if the gene is used as cutoff point P.value: estimated p-value for each gene
两个表与gene.index鉴定基因:基因指数在集反相/ Rsum的原始数据:计算机等级产品/每个基因的功能界别的总和:(class1/class2):表达类倍1/2级。亲民党:估计亲民党对每一个基因,如果基因被用来作为分界点P.value:每个基因的估计p值
Table 1 list genes that are up-regulated under class 2, Table 1 ist genes that are down-regulated under class 2,
表1列出下,表2级上调北京时间1下2级下调基因的基因,
作者(S)----------Author(s)----------
Fangxin Hong <a href="mailto:fhong@salk.edu">fhong@salk.edu</a>
参考文献----------References----------
P.(2004) Rank Products: A simple, yet powerful, new method to detect differentially regulated genes in replicated microarray experiments, FEBS Letter, 57383-92
参见----------See Also----------
plotRP RP RPadvance RSadvance
plotRPRPRPadvanceRSadvance
举例----------Examples----------
# Load the data of Golub et al. (1999). data(golub) [Golub等装入的数据。 (1999年)。数据(戈卢布)]
# contains a 3051x38 gene expression[包含一个的3051x38基因表达]
# matrix called golub, a vector of length called golub.cl [矩阵称为戈卢布,向量的长度称为golub.cl]
# that consists of the 38 class labels,[由38类标签,]
# and a matrix called golub.gnames whose third column [矩阵称为其第三列golub.gnames]
# contains the gene names.[包含的基因名称。]
data(golub)
#use a subset of data as example, apply the rank [使用数据的一个子集为例,申请排名]
#product method[产品的方法]
subset <- c(1:4,28:30)
#Setting rand=123, to make the results reproducible,[设置兰特= 123,结果重现性好,]
#identify genes [确定基因]
RP.out <- RP(golub[,subset],golub.cl[subset],rand=123)
#get two lists of differentially expressed genes [得到两个差异表达的基因列表]
#by setting FDR (false discivery rate) =0.05[通过设置FDR(虚假discivery率)= 0.05]
table=topGene(RP.out,cutoff=0.05,method="pfp",logged=TRUE,logbase=2,
gene.names=golub.gnames[,3])
table$Table1
table$Table2
#using pvalue<0.05[使用pvalue <0.05]
topGene(RP.out,cutoff=0.05,method="pval",logged=TRUE,logbase=2,
gene.names=golub.gnames[,3])
#by selecting top 10 genes[选择前10个基因]
topGene(RP.out,num.gene=10,gene.names=golub.gnames[,3])
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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