normQpcrRankInvariant(qpcrNorm)
normQpcrRankInvariant()所属R语言包：qpcrNorm
Function for Rank-Invariant Set Normalization for qPCR Data.
排名不变qPCR数据集标准化的功能。
译者：生物统计家园网 机器人LoveR
描述----------Description----------
Implements rank-invariant set normalization for a qpcrBatch object. We have adapted this algorithm from the function normalize.invariantset from the affy package.
实现qpcrBatch对象的排名不变集的标准化。我们已经适应了这种算法从功能normalize.invariantsetaffy包。
用法----------Usage----------
normQpcrRankInvariant(qBatch, refType, rem.highCt = FALSE, thresh.Ct = 30)
参数----------Arguments----------
参数：qBatch
A qpcrBatch object.
一个qpcrBatch对象。
参数：refType
Indicates what reference sample should be used, can be an integer or character string. See Details below.
表示应使用什么样的参考样本，可以是一个整数或字符串。详见下文。
参数：rem.highCt
Logical indicator, TRUE if user wishes to remove genes with high Ct values (very low expression) that may be associated poor data quality.
逻辑指示灯，TRUE，如果用户希望删除高Ct值（极低表达）可能相关的数据质量差的基因。
参数：thresh.Ct
Numerical value indicating the Ct value cutoff threshold, if rem.highCt = FALSE, genes with Ct values > thresh.Ct are removed from the data set.
显示Ct值临界阈值的数值，如果rem.highCt= FALSE时，基因的Ct值> thresh.Ct从数据集删除。
Details
详情----------Details----------
The algorithm computes all rank-invariant sets of genes between pairwise comparisons where each experimental sample in the qpcrBatch object is paired against a reference. There are several ways to specify what a sensible choice for the reference sample should be. <br> <br> 1. The reference is an experimental sample in the qpcrBatch object. <br> Specify refType as an integer value, corresponding to the index of which experimental sample is the reference. <br> <br> 2. The reference is the sample which is closest to mean of all the experiments. <br> Specify refType = "mean". <br> <br> 3. The reference is the sample which is closest to median of all the experiments. <br> Specify refType = "median". <br> <br> 4. The reference is the mean of all experiments in the qpcrBatch object. <br> Specify refType = "pseudo.mean". <br> <br> 5. The reference is the median of all experiments in the qpcrBatch object. <br> Specify refType = "pseudo.median". <br>
该算法计算所有基因的排名不变集之间的成对比较实验样本，其中每个qpcrBatch对象是针对一个参考配对。有几种方式来指定参考样本的一个明智的选择应该是什么。参考参考1。引用是在qpcrBatch对象的实验样品。参考指定refTypeinteger值，相应的实验样品是参考指数。参考参考2。参考最接近意味着所有的实验样品。参考指定refType = "mean"。参考参考3。参考样品，这是最接近的所有实验中位数。参考指定refType = "median"。 <BR> <BR> 4。参考是平均在qpcrBatch所有的实验对象。参考指定refType = "pseudo.mean"。参考参考5。参考是在qpcrBatch所有的实验对象的中位数。参考指定refType = "pseudo.median"。参考
值----------Value----------
A qpcrBatch object, the normalized slot is now set at TRUE. The names of the rank-invariant genes used for normalization are stored as a vector in the normGenes slot of the qpcrBatch object returned. To retrieve the rank-invariant gene names, use qpcrBatch@normGenes.
一个qpcrBatch对象，normalized插槽设置为TRUE。标准化的排名不变的基因的名称存储在normGenes返回的对象的插槽qpcrBatch向量。要检索的排名不变的基因名称，使用qpcrBatch@normGenes。
作者（S）----------Author(s)----------
Jess Mar <a href="mailto:jess@jimmy.harvard.edu">jess@jimmy.harvard.edu</a>
参见----------See Also----------
normQpcrQuantile, normalize.invariantset
normQpcrQuantile，normalize.invariantset
举例----------Examples----------
data(qpcrBatch.object)
mynormRI.data <- normQpcrRankInvariant(qpcrBatch.object, 1)
mynormRI.data@normGenes # retrieves names of genes in the rank-invariant set[基因名称检索中排名不变集]
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注：
注1：为了方便大家学习，本文档为生物统计家园网机器人LoveR翻译而成，仅供个人R语言学习参考使用，生物统计家园保留版权。
注2：由于是机器人自动翻译，难免有不准确之处，使用时仔细对照中、英文内容进行反复理解，可以帮助R语言的学习。
注3：如遇到不准确之处，请在本贴的后面进行回帖，我们会逐渐进行修订。
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发表于 2012-2-26 11:37:44
