ampliconPrediction(MassArray)
ampliconPrediction()所属R语言包:MassArray
Amplicon prediction
扩增预测
译者:生物统计家园网 机器人LoveR
描述----------Description----------
Function to predict amplicon fragmentation pattern and details for T&C reactions on the plus and minus strands
函数T&C反应扩增破碎化格局和细节上的加号和减号股预测
用法----------Usage----------
ampliconPrediction(sequence, lower.threshold = 1500, upper.threshold = 7000, fwd.tag = "AGGAAGAGAG", rev.tag = "AGCCTTCTCCC", plot = TRUE, table = TRUE, lwd = 1, cex = 1, multiple.conversion = FALSE)
参数----------Arguments----------
参数:sequence
Nucleotide sequence input as a character string
核苷酸序列输入字符串
参数:lower.threshold
Lower limit (in Da) of usable mass window (default: 1500)
可用质量窗口下限(大)(默认是:1500)
参数:upper.threshold
Upper limit (in Da) of usable mass window (default: 7000)
可用的质量窗口(大)的上限(默认是:7000)
参数:fwd.tag
Nucleotide tag sequence 5' of the forward primer
核苷酸标签序列的正向引物5
参数:rev.tag
T7-containing nucleotide tag sequence 5' of the reverse primer
含T7 - 核苷酸标记序列的反向引物5
参数:plot
Logical specifying whether or not to display graphical representation of fragmentation profiles (default is TRUE)
逻辑指定是否显示碎片型材图形表示(默认是TRUE)
参数:table
Logical specifying whether or not to return tabular representation of fragmentation profiles (default is TRUE)
逻辑指定是否返回碎片型材表格形式(默认是TRUE)
参数:lwd
The line width used for fragmentation display, a positive number, defaulting to 1
用于碎片显示,线宽为正数,默认1
参数:cex
A numerical value (defaulting to 1) giving the amount by which plotting text and symbols should be magnified relative to the default
一个数值(默认为1)给予的数额由绘制的文本和符号相对应放大默认
参数:multiple.conversion
Logical value specifying whether or not to include multiple CGs on the same conversion control fragment where possible (default is FALSE).
逻辑值,指明是否包括多个相同的转换控制片段,在可能的情况下(默认为FALSE)CGS。
Details
详情----------Details----------
Plotted fragmentation patterns contain a number of detailed features including: CG positions, molecular weight overlaps, conversion controls, fragment assayability, and more.
绘制碎片模式,包含详细的功能,包括:重心位置,分子量重叠,转换控制,assayability片段,以及更多。
Note that the graphical output does not contain a built-in legend at this time, but the plot may be interepreted as follows: Putative fragmentation patterns are shown for T and C-cleavage reactions on both the plus and minus strands of an input amplicon, with the T-forward, T-reverse, C-forward, and C-reverse shown in descending order. CG dinucleotides (filled circles) are numbered and colored in blue. Other fragments are colored according to their ability to be assayed: fragment molecular weight outside the testable mass window (gray), fragment molecular weight overlapping with another fragment (red), fragment containing a potential conversion control (green), or fragment uniquely assayable but containing no CGs (black). Linked arrowheads denote molecular weight overlaps between multiple CG-containing fragments. Yellow highlights represent tagged or primer sequences, while lavender highlights denote user-specified "required" sites.
注意图形输出中不包含一个内置的传说,但此时的图,可作为interepreted如下:假定碎片图案的T-裂解反应显示输入扩增的加号和减号股, T型,T型反向的C-向前,和C-扭转降序显示。编号的CG二核苷酸(实心圆)和蓝色。其他碎片的颜色根据自己的能力进行检测:片段的分子量测试的质量窗口(灰色)外,另一个片段(红色)重叠的片段分子量,片段含有一个潜在的转换控制(绿色),或片段唯一assayable不包含CGS(黑)。链接箭头表示分子量多个含重心片段之间的重叠。黄色亮点代表标签或引物序列,而薰衣草的亮点表示用户指定的“所需”的网站。
值----------Value----------
If table is TRUE, returns a list containing the following items:
如果table是TRUE,返回一个列表,其中包含下列项目:
参数:summary
A summary matrix of logical values specifying whether or not each CG is assayable by a given combination of cleavage reaction and DNA strand
摘要矩阵的逻辑值,指定是否每个企业管治是由裂解反应和DNA结合assayable
参数:counts
A numerical tally of the quantity of CGs that are assayable by each assay
每个检测assayable一个的CGS数量的数值相符
作者(S)----------Author(s)----------
Reid F. Thompson (<a href="mailto:rthompso@aecom.yu.edu">rthompso@aecom.yu.edu</a>), John M. Greally (<a href="mailto:jgreally@aecom.yu.edu">jgreally@aecom.yu.edu</a>)
举例----------Examples----------
ampliconPrediction("TGGAACACCCAGCAAAGATCAAGCAGGAAAGGGCGCACGCAGCCTTCGTTGCTAACCTCCTCTGGACTCTGGTACCCCAGGCACCGCGAATGCTCCCCACCTCAGCCCCCTGACCTTTACCATCGCTGAAGCGGGCGTCGCTGATGTCTGCGGCGAGCCTGCCGACCAGCCCAGCTGCCCAGAGGAGCAGCCAGGCAAGGGCGCTGGCAGCCAGGACGCCGGAGCCCGACGCCCGAGAGGGGCGCGCGGAGCAAGCTGCGGTCACGGGAGGAACCTGAGCACGCAGAGCGTACCCCCACCTTCCACGGTGACCCGGACAGAACGCTCCTTGCGCTCCCACCCTAGGACCCCCTGTAACTCCAGGTTCCTGAGA")
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
注1:为了方便大家学习,本文档为生物统计家园网机器人LoveR翻译而成,仅供个人R语言学习参考使用,生物统计家园保留版权。
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