multipleAlignment-class(flagme)
multipleAlignment-class()所属R语言包:flagme
Data Structure for multiple alignment of many GCMS samples
许多GCMS样品的多序列比对的数据结构
译者:生物统计家园网 机器人LoveR
描述----------Description----------
Store the raw data and optionally, information regarding signal peaks for a number of GCMS runs
存储的原始数据,并有选择地,有关信号峰值为GCMS运行数
用法----------Usage----------
multipleAlignment(pd,group,bw.gap=0.8,wn.gap=0.6,bw.D=.20,wn.D=.05,filterMin=3,lite=FALSE,usePeaks=TRUE,df=50,verbose=TRUE,timeAdjust=FALSE,doImpute=FALSE)
参数----------Arguments----------
参数:pd
a peaksDataset object
peaksDataset对象
参数:group
factor variable of experiment groups, used to guide the alignment algorithm
因子变量实验组,用于指导对齐算法
参数:bw.gap
gap parameter for "between" alignments
“之间的”路线的差距参数
参数:wn.gap
gap parameter for "within" alignments
“内”路线的差距参数
参数:bw.D
distance penalty for "between" alignments
距离刑罚“之间的”路线
参数:wn.D
distance penalty for "within" alignments
“内”路线的距离刑罚
参数:filterMin
minimum number of peaks within a merged peak to be kept in the analysis
在合并后的高峰要保持在最低峰分析
参数:lite
logical, whether to keep "between" alignment details (default, FALSE)
逻辑,是否保留“之间的”对齐的细节(默认FALSE)
参数:usePeaks
logical, whether to use peaks (if TRUE) or the full 2D profile alignment (if FALSE)
逻辑,是否使用峰(TRUE)或全二维轮廓对齐(如果FALSE)
参数:df
distance from diagonal to calculate similarity
从对角线的距离来计算相似
参数:verbose
logical, whether to print information
逻辑,是否要打印的信息
参数:timeAdjust
logical, whether to use the full 2D profile data to estimate retention time drifts (Note: time required)
逻辑,是否使用完整的二维轮廓数据来估计的保留时间漂移(注:所需的时间)
参数:doImpute
logical, whether to impute the location of unmatched peaks
逻辑,是否意指无与伦比的峰的位置
Details
详情----------Details----------
multipleAlignment is the data structure giving the result of an alignment across several GCMS runs.
multipleAlignment是数据结构的使跨越几个GCMS运行路线的结果。
Multiple alignments are done progressively. First, all samples with the same tg$Group label with be aligned (denoted a "within" alignment). Second, each group will be summarized into a pseudo-data set, essentially a spectrum and retention time for each matched peak of the within-alignment. Third, these "merged peaks" are aligned in the same progressive manner, here called a "between" alignment.
逐步完成了多个路线。首先,所有样品相同tg$Group标签对齐(表示“在”对齐)。其次,每个组将被归纳为一个伪数据集,基本上是为每个匹配的内对齐峰的保留时间和频谱。第三,这些“合并后的山峰”排列在同一循序渐进的方式,这里被称为“之间的”对齐。
值----------Value----------
multipleAlignment object
multipleAlignment对象
作者(S)----------Author(s)----------
Mark Robinson
参考文献----------References----------
参见----------See Also----------
peaksDataset, betweenAlignment, progressiveAlignment
peaksDataset,betweenAlignment,progressiveAlignment
举例----------Examples----------
require(gcspikelite)
# paths and files[路径和文件]
gcmsPath<-paste(.find.package("gcspikelite"),"data",sep="/")
cdfFiles<-dir(gcmsPath,"CDF",full=TRUE)
eluFiles<-dir(gcmsPath,"ELU",full=TRUE)
# read data, peak detection results[读取数据,峰值检测结果]
pd<-peaksDataset(cdfFiles[1:2],mz=seq(50,550),rtrange=c(7.5,8.5))
pd<-addAMDISPeaks(pd,eluFiles[1:2])
# multiple alignment[多序列比对]
ma<-multipleAlignment(pd,c(1,1),wn.gap=0.5,wn.D=.05,bw.gap=0.6,bw.D=.2,usePeaks=TRUE,filterMin=1,df=50,verbose=TRUE)
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注:
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