read.HTSeqCounts(DEXSeq)
read.HTSeqCounts()所属R语言包:DEXSeq
Read counts output from HTSeq script.
读计数HTSeq脚本的输出。
译者:生物统计家园网 机器人LoveR
描述----------Description----------
This function reads the output files from the HTSeq python scripts dexseq_prepare_annotation.py and dexseq_count.py and gives back an ExonCountSet object.
此功能读取,从HTSeq python脚本dexseq_prepare_annotation.py和dexseq_count.py输出文件和,还给ExonCountSet对象。
用法----------Usage----------
read.HTSeqCounts(countfiles, design, flattenedfile=NULL)
参数----------Arguments----------
参数:countfiles
A string vector containing the output files with the paths from dexseq_count.py.
一个字符串向量包含与从dexseq_count.py路径的输出文件。
参数:design
A vector of factors with information corresponding to each of the countfiles or a data frame design (each column with a factor and each row with its respective sample. If strings are given, they will be converted to factors.
一个因素,相应的countfiles或数据框设计(每一个因素,每一个都有其各自的样本行列。如果字符串,它们将被转换因素与信息的向量。
参数:flattenedfile
An flattened annotation gtf file generated by dexseq_prepare_annotation.py. It is necessary for the visualization of the data but not required to test for alternative exon usage.
一个扁平的注解GTF dexseq_prepare_annotation.py产生的文件。这是必要的数据可视化,但不是必需的测试使用替代外显子。
值----------Value----------
An ExonCount object.
一个ExonCount的对象。
举例----------Examples----------
library(DEXSeq)
inDir = system.file("extdata", package="pasilla", mustWork=TRUE)
annotationfile = file.path(inDir, "Dmel.BDGP5.25.62.DEXSeq.chr.gff")
samples = data.frame(
condition = c(rep("treated", 3), rep("untreated", 4)),
replicate = c(1:3, 1:4),
row.names = dir(system.file("extdata", package="pasilla", mustWork=TRUE),
pattern="fb.txt"),
stringsAsFactors = TRUE,
check.names = FALSE
)
annotationfile = file.path(inDir, "Dmel.BDGP5.25.62.DEXSeq.chr.gff")
## Not run: [#无法运行:]
ecs = read.HTSeqCounts(countfiles = file.path(inDir, rownames(samples)),
design = samples,
flattenedfile = annotationfile)
## End(Not run)[#结束(不运行)]
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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