找回密码
 注册
查看: 626|回复: 0

R语言 cellHTS包 readPlateData()函数中文帮助文档(中英文对照)

[复制链接]
发表于 2012-2-25 14:21:58 | 显示全部楼层 |阅读模式
readPlateData(cellHTS)
readPlateData()所属R语言包:cellHTS

                                        Read a collection of plate reader data files
                                         阅读酶标仪数据文件的集合

                                         译者:生物统计家园网 机器人LoveR

描述----------Description----------

Reads a collection of plate reader data files into a data.frame. The names of the files, plus additional information (plate number, repeat number) is expected in a tab-delimited table specified by the argument x.  
读入数据框酶标仪数据文件的集合。预计在由参数x指定制表符分隔的表文件的名称,再加上额外的信息(车牌号码,重复次数)。


用法----------Usage----------


readPlateData(filename, path=dirname(filename), name, importFun, verbose=TRUE, plateType)



参数----------Arguments----------

参数:filename
the name of the file table (see details). This argument is just passed on to the read.table function, so any of the valid argument types for read.table are valid here, too.
文件表的名称(见详情)。这种说法只是通过read.table功能,所以任何有效的参数类型为read.table是有效的,在这里,太。


参数:name
a character of length 1 with the experiment name.
1,实验名称长度的字符。


参数:path
a character of length 1 indicating the path in which to find the plate reader files. By default,  it can extract the path from filename.
一个长度为1的字符显示在其中找到酶标仪文件路径。默认情况下,它可以从filename提取的路径。


参数:importFun
a function that should be used to read each plate result file. The default function works for plate reader data files. See details.
一个应该被用来阅读每盘结果文件的功能。工程酶标仪数据文件的默认功能。查看详情。


参数:verbose
a logical value, if TRUE, the function reports some of its intermediate progress.
一个逻辑值,如果属实,功能及其中间体的进展报告。


参数:plateType
(deprecated argument) a character of length 1 giving the format of the plate: "96" for  96-well plate format, or "384" for a 384-well plate format.
(过时的说法)一个字符的长度1给该板块的格式:“96”为96孔板格式,或“384”384孔板格式。


Details

详情----------Details----------

The file table is expected to be a tab-delimited file with at least three columns, and column names Filename, Plate, and Replicate. The contents of the columns Plate and Replicate are expected to be integers. Further columns are allowed.
文件表预计将用制表符分隔的文件至少有三列,列名Filename,Plate,Replicate。列的内容Plate和Replicate预计将整数。进一步列是允许的。

We distinguish between plates and plate result file. A plate result file contains the measurements results for all replicates and all channels of a plate, which is the physical carrier of the reagents.
我们区分板和板的结果文件。 A板的结果文件中包含的所有复制和一个盘子,这是物理向量试剂所有通道的测量结果。

importFun can be used to define other functions to import other data files, such as flow cytometry data files, etc. The importFun function should receive as an input the name of a result plate file to read, and return a list with two components:
importFun可以用来定义等功能导入其他数据文件,如流式单元仪数据文件等importFun函数作为输入接收结果盘文件读取的名称,返回两部分组成的一个列表:

The first component should be a 'data.frame' with the following slots:
第一部分应该是一个“数据框”具有以下插槽:

well, a character vector with the well identifier in the plate.
well,以及在板标识与特征向量。

val, the intensity values measured at each well.
val,每口井的强度值测量。

The second component of this list should be a character vector containing a copy of the imported input data file (such as the output of readLines). It should be suitable to be used as input for writeLines, since it will be used to reproduce the intensity files that are linked in the HTML quality reports generated by writeReport.
此列表的第二个组成部分,应该是一个特征向量,包含进口的输入数据文件副本(如输出readLines)。它应该是适合用作为writeLines的输入,因为它会被用来重现强度链接文件是在质量writeReport生成的HTML报告。

For example, to import plate data files from EnVision plate reader, set importFun=getEnVisionRawData or importFun=getEnvisionCrosstalkCorrectedData. See function getEnVisionRawData.
例如,进口板的数据文件,从设想酶标仪,设置importFun=getEnVisionRawData或importFun=getEnvisionCrosstalkCorrectedData。见功能getEnVisionRawData。


值----------Value----------

An object of class "cellHTS", which is currently implemented as a list with elements
一个对象的类"cellHTS",目前正在实施的元素的列表


参数:name
copy of the input argument name
输入参数name副本


参数:xraw
an array of dimension plateSize x number of plates x number of replicates x number of channels, containing the imported measurement data.
板数量x的维plateSize的X号的数组复制的X通道的数量,包含进口的测量数据。


参数:pdim
a numeric vector of length 2 containing the number of rows and columns in a plate. The product of these two numbers is the first dimension of xraw. This corresponds to the plate             format used in the screen, and it is automatically determined from the plate result files. The allowed formats are 96-well  or 384-well plates.  
数值向量的长度为2包含一个板块中的行和列的数目。这两个数字的产品是第一维xraw。这相当于在屏幕上使用的格式板,它会自动从盘的结果文件确定。允许的格式是96或384孔板。


参数:batch
an integer vector with the batch number (1, 2, ...) for each plate. Its length corresponds to the second dimension of  of xraw.
一个批号,每盘(1,2,...)的整数向量。其长度相当于第二维xraw。


参数:plateList
a data.frame containing what was read from input file x, plus a column status of type character: it contains the string "OK" if the data import appeared to have gone well, and the respective error or warning message otherwise.
数据框,其中包含从读取输入文件x,加列status型性格:它包含字符串“OK”,如果出现数据导入顺利,各自否则错误或警告消息。


参数:intensityFiles
a list, where each component contains a copy of the imported input data files. Its length corresponds to the number of rows of plateList.
一个列表,其中每个组件包含了进口输入数据文件副本。它的长度相当于plateList行的数目。


参数:state
a logical vector representing the processing status of the object.
一个逻辑向量代表该对象的处理状态。


作者(S)----------Author(s)----------


W. Huber <a href="mailto:huber@ebi.ac.uk">huber@ebi.ac.uk</a>, Ligia Braz <a href="mailto:ligia@ebi.ac.uk">ligia@ebi.ac.uk</a>



参见----------See Also----------

getEnVisionRawData
getEnVisionRawData


举例----------Examples----------


    datadir <- system.file("KcViabSmall", package = "cellHTS")
    x <- readPlateData("Platelist.txt", "KcViabSmall", path=datadir)

    ## To read data files obtained from an EnVision plate reader:[#读取与设想酶标仪获得的数据文件:]
    datadir <- system.file("EnVisionExample", package = "cellHTS")
    x <- readPlateData("platelist.txt", "EnVisionEx",
          importFun=getEnVisionRawData, path=datadir)

       ## to get the cross talk corrected data:[#相声修正后的数据:]
       y <- readPlateData("platelist.txt", "EnVisionEx",
           importFun=getEnVisionCrosstalkCorrectedData, path=datadir)


转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。


注:
注1:为了方便大家学习,本文档为生物统计家园网机器人LoveR翻译而成,仅供个人R语言学习参考使用,生物统计家园保留版权。
注2:由于是机器人自动翻译,难免有不准确之处,使用时仔细对照中、英文内容进行反复理解,可以帮助R语言的学习。
注3:如遇到不准确之处,请在本贴的后面进行回帖,我们会逐渐进行修订。
回复

使用道具 举报

您需要登录后才可以回帖 登录 | 注册

本版积分规则

手机版|小黑屋|生物统计家园 网站价格

GMT+8, 2025-1-27 13:41 , Processed in 0.021428 second(s), 16 queries .

Powered by Discuz! X3.5

© 2001-2024 Discuz! Team.

快速回复 返回顶部 返回列表