compensate(plateCore)
compensate()所属R语言包:plateCore
Compensate a flowPlate to correct for the effects of spillover.
补偿flowPlate纠正外溢的影响。
译者:生物统计家园网 机器人LoveR
描述----------Description----------
Flow samples are often stained with multiple types of fluorophores. Unfortunately, the emission spectra for these different fluorophores often overlap, and the signals must be corrected before proceeding with the analysis. Compensate adjusts for spillover using the method implemented in the package flowCore. Unlike flowCore, compensate only adjusts for the dyes/fluorophores listed in wellAnnotation.
流动样品往往与多种类型的荧光染色。不幸的是,这些不同的荧光发射光谱往往重叠,并进行分析的信号之前,必须纠正。补偿调节外溢使用在包flowCore实施的方法。不像flowCore,补偿只列出染料/ wellAnnotation的荧光调整。
用法----------Usage----------
## S4 method for signature 'flowPlate,ANY'
compensate(x, spillover)
参数----------Arguments----------
参数:x
A flowPlate
一个flowPlate
参数:spillover
The compensation matrix where the row and column names match the fluorescence channels of the flowPlate.
补偿矩阵的行和列的名称匹配flowPlate的荧光通道。
值----------Value----------
Returns a compensated flowPlate.
返回一个补偿flowPlate。
作者(S)----------Author(s)----------
Errol Strain
参见----------See Also----------
See Also compensation-class
另见compensation-class
举例----------Examples----------
library(plateCore)
data(plateCore)
# Create the compensation matrix[建立补偿矩阵]
comp.mat <- spillover(x=compensationSet,unstained=sampleNames(compensationSet)[5],
patt=".*H",fsc="FSC-H",ssc="SSC-H",method="median")
## Get the lymphocytes[#获取淋巴单元]
rectGate <- rectangleGate("FSC-H"=c(300,700),"SSC-H"=c(50,400))
pbmcPlate <- Subset(pbmcPlate, rectGate)
# Create a flowPlate from the sample data in plateCore[从在plateCore的样本数据,创建1 flowPlate]
fp <- flowPlate(pbmcPlate,wellAnnotation,plateName="P1")
# apply the compensation matrix[申请补偿矩阵]
fp <- compensate(fp,comp.mat)
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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