Implementation of the Rank Product method for the detection of alternative splicing events
等级产品的剪接事件的检测方法的实施
译者:生物统计家园网 机器人LoveR
描述----------Description----------
This is a graphical interface to the RP function from RankProd package applied to detection of alternative splicing
这是一个图形界面,应用剪接检测RankProd包到RP功能
用法----------Usage----------
erankProdAltSpl()
Details
详情----------Details----------
Before using this method it is strongly suggested to perform a filter on the basis of DABG p-values using the filtering function available in the filtering menu. DABG values can be calculated if exon array probe set data are generated using the oneChannelGUI graphical implementation to APT tools. Affymetrix suggests to calculate probe set intensity at gene level using iterPlier and at exon level using plier. Subsequently SpliceIndex need to be calculated using the function available in the exon menu. Finally the Rank Product method could be applied exon by exon. For more details on the method see RankProd package. Selection of putative alternative splicing could be done using the filtering function available in the filtering menu of
使用这种方法之前,强烈建议P-值使用的过滤功能,可在过滤菜单DABG的基础上执行一个过滤器。 DABG值可以计算出,如果外显子阵列探测数据集生成使用图形oneChannelGUI实施APT的工具。 Affymetrix公司的基因使用iterPlier水平和外显子水平,用钳子建议计算探针组强度。随后SpliceIndex需要使用功能外显子菜单中可用来计算。最后排名产品的方法可以适用于外显子外显子。为更多细节上的方法看到RankProd的包。假定剪接的选择,可以使用过滤功能,可在过滤菜单
注意----------Note----------
IMPORTANT we are still evaluating the efficacy of this method for detection of alternative splicing events. Use it being concious of this!
重要的是我们仍在评估剪接事件检测这一方法的有效性。它使用这个concious!