constructBins(mosaics)
constructBins()所属R语言包:mosaics
Construct bin-level ChIP-sep data from an aligned read file
从对齐的读文件构建斌级芯片九月数据
译者:生物统计家园网 机器人LoveR
描述----------Description----------
Preprocess and construct bin-level ChIP-sep data from an aligned read file.
预处理和建造的bin级芯片九月数据对齐的读文件。
用法----------Usage----------
constructBins( infileLoc=NULL, infileName=NULL, fileFormat=NULL, outfileLoc=infileLoc,
byChr=FALSE, fragLen=200, binSize=fragLen, capping=0, perl = "perl" )
参数----------Arguments----------
参数:infileLoc
Directory of the aligned read file to be processed.
对齐的读文件目录进行处理。
参数:infileName
Name of the aligned read file to be processed.
对齐的读文件的名称来进行处理。
参数:fileFormat
Format of the aligned read file to be processed. Currently, constructBins permits the following aligned read file formats: "eland_result" (Eland result), "eland_extended" (Eland extended), "eland_export" (Eland export), "bowtie" (default Bowtie), "sam" (SAM), and "bed" (BED).
对齐的读文件的格式进行处理。目前,constructBins允许不对齐的读下列文件格式:"eland_result"(伊兰结果),"eland_extended"(伊兰扩展),"eland_export"(伊兰出口),"bowtie" (默认的蝴蝶结),"sam"(SAM)和"bed"(床)。
参数:outfileLoc
Directory of processed bin-level files. By default, processed bin-level files are exported to the directory that the aligned read file is located.
处理滨级的文件目录。默认情况下,处理的bin级文件远销对齐的读文件位于目录。
参数:byChr
Construct separate bin-level file for each chromosome? Possible values are TRUE or FALSE. If byChr=FALSE, all chromosomes are exported into one file. Default is FALSE.
每个染色体构建独立的bin级别的文件?可能的值是TRUE或FALSE。如果byChr=FALSE,所有的染色体都导出到一个文件中。默认FALSE。
参数:fragLen
Average fragment length. Default is 200.
平均片段长度。默认是200。
参数:binSize
Size of bins. By default, bin size equals to fragLen (average fragment length).
箱的大小。默认情况下,箱大小等于fragLen(平均片段长度)。
参数:capping
Maximum number of reads allowed to start at each nucleotide position. To avoid potential PCR amplification artifacts, the maximum number of reads that can start at a nucleotide position is capped at capping. Capping is not applied if non-positive capping is used. Default is 0 (no capping).
读取允许的最大数量,开始在每个核苷酸的位置。为了避免潜在的PCR扩增文物,在核苷酸位置,就可以开始读取的最大数量的上限是在capping。不适用,如果非正capping用于封顶。默认为0(没有上限)。
参数:perl
Name of the perl executable to be called. Default is "perl".
被称为Perl的可执行文件的名称。默认"perl"。
Details
详情----------Details----------
Bin-level files are constructred from the aligned read file and exported to outfileLoc. If byChr=FALSE, bin-level files are named as [infileName]_fragL[fragLen]_bin[binSize].txt, If byChr=TRUE, bin-level files are named as [chrID]_[infileName]_fragL[fragLen]_bin[binSize].txt, where [chrID] is chromosome ID that reads align to. These chromosome IDs are extracted from the aligned read file. Constructed bin-level files can be loaded into the R environment using the method readBins.
斌级文件对齐的读文件从constructred出口outfileLoc。如果byChr=FALSE,宾级的文件被命名为[infileName]_fragL[fragLen]_bin[binSize].txt如果byChr=TRUE斌级文件[chrID]_[infileName]_fragL[fragLen]_bin[binSize].txt,其中[chrID]是染色体的ID命名读取对齐。这些染色体的ID提取对齐的读文件。构建斌级文件可以加载到R的环境中使用的方法readBins。
constructBins currently supports the following aligned read file formats: Eland result ("eland_result"), Eland extended ("eland_extended"), Eland export ("eland_export"), default Bowtie ("bowtie"), SAM ("sam"), and BED ("bed"). This method assumes that these aligned read files are obtained from single-end tag (SET) experiments and retains only reads mapping uniquely to the reference genome.
constructBins目前支持对齐的读文件格式如下:的伊兰结果("eland_result"),:伊兰扩展("eland_extended"),:伊兰出口("eland_export"),默认领结(<X >),SAM("bowtie"),床("sam")。此方法假定,从单端标签(套)实验获得这些对齐的读文件,并保留只读取映射唯一的参考基因组。
值----------Value----------
Processed bin-level files are exported to the directory specified in outfileLoc.
斌级处理的文件导出到指定的目录outfileLoc。
作者(S)----------Author(s)----------
Dongjun Chung, Pei Fen Kuan, Sunduz Keles
参考文献----------References----------
"A Statistical Framework for the Analysis of ChIP-Seq Data", Journal of the American Statistical Association, Vol. 106, pp. 891-903.
参见----------See Also----------
readBins, BinData.
readBins,BinData。
举例----------Examples----------
## Not run: [#无法运行:]
constructBins( infileLoc="/scratch/eland/",
infileName="STAT1_eland_results.txt",
fileFormat="eland_result", outfileLoc=infileLoc,
byChr=FALSE, fragLen=200, binSize=fragLen, capping=0 )
## End(Not run)[#结束(不运行)]
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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