identifySNPs(MassArray)
identifySNPs()所属R语言包:MassArray
Identify SNPs
识别单核苷酸多态性
译者:生物统计家园网 机器人LoveR
描述----------Description----------
Function to identify potential single nucleotide polymorphisms (SNPs) which allow mapping of a novel peak sequence to the expected amplicon sequence
功能,以确定潜在的单核苷酸多态性(SNP),使预期的扩增序列映射到一个新的高峰序列
用法----------Usage----------
identifySNPs(peak.sequence, sequence, rxn = c("T", "C"))
参数----------Arguments----------
参数:peak.sequence
Nucleotide sequence (can also be base composition - ex: "A6G2C1T3") as a character string
作为一个字符串的核苷酸序列(也可以是碱基组成 - 例如:“A6G2C1T3”)
参数:sequence
Nucleotide sequence for wildtype/expected amplicon as a character string
核苷酸序列为野生型/字符串预期的扩增
参数:rxn
One of "T" or "C" specifying which cleavage reaction to use for SNP analysis
一个“T”型或“C”指定用于SNP分析裂解反应
Details
详情----------Details----------
The algorithm steps through the sequence, substituting one nucleotide at a time with the other three base pairs or a blank character (deletion), in order to determine a base compositional match to the input peak.sequence which represents a peak not found in the native sequence.
通过sequence代核苷酸在时间与其他3个碱基对或一个空白字符(删除),以该算法的步骤,以确定输入peak.sequence这代表了一个基本的作曲比赛峰没有发现本地sequence。
值----------Value----------
Returns a list of potential SNP matches for the input peak.sequence. Each element of the list contains multiple items as follows:
返回输入peak.sequence潜在的SNP比赛名单。列表中的每个元素都包含多个项目如下:
参数:sequence
corresponds to peak.sequence
对应peak.sequence
参数:position
corresponds to the matched position within sequence
对应的匹配的位置在sequence
参数:base
corresponds to the altered nucleotide (i.e. "A", "T", "C", "G", or "")
对应于核苷酸的改变(即“一”,“T”型,“C”“G”或“”)
参数:type
corresponds to the class of SNP (i.e. "substitution" or "deletion")
对应的类的SNP(即“替代”或“删除”)
作者(S)----------Author(s)----------
Reid F. Thompson (<a href="mailto:rthompso@aecom.yu.edu">rthompso@aecom.yu.edu</a>), John M. Greally (<a href="mailto:jgreally@aecom.yu.edu">jgreally@aecom.yu.edu</a>)
举例----------Examples----------
## SINGLE SUBSTITUTION[#单一替代]
identifySNPs("AAGT","AATTTT")
## MULTIPLE SUBSTITUTION POSSIBILITIES[#多替代的可能性]
identifySNPs("A1G1T1","AATTTT")
## DELETION[#删除]
identifySNPs("AAT","AGATTTT")
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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