summarizeReplicates(cellHTS2)
summarizeReplicates()所属R语言包:cellHTS2
Summarize between scored replicate values given in a cellHTS object to obtain a single value for each probe
总结之间打进在cellHTS对象的复制值,获得每个探针的单值
译者:生物统计家园网 机器人LoveR
描述----------Description----------
This function summarizes the replicate values stored in slot assayData of a cellHTS object and calculates a single score for each probe. Data are stored in slot assayData overridding its current content.
此功能总结插槽中存储的复制值assayDatacellHTS对象的,并计算出每个探针的单节得分。数据存储在插槽assayData的overridding其当前内容。
Currently this function is implemented only for single-color data.
目前此功能只为单一颜色的数据。
用法----------Usage----------
summarizeReplicates(object, summary ="min")
参数----------Arguments----------
参数:object
an object of class cellHTS that has already been normalized and scored (see details).
一个类的对象cellHTS已经标准化,并取得(见详情)。
参数:summary
a character string indicating how to summarize between replicate measurements. One of "min" (default), "mean", "median", "max", "rms", "closestToZero", or "FurthestFromZero" can be used (see details).
一个字符串,指示如何总结之间的重复测量。一个“分”(默认),“是什么意思”,“中位数”,“最大”,“RMS”,“closestToZero”,或“FurthestFromZero”可以使用(见详情)。
Details
详情----------Details----------
A single value per probe is calculated by summarizing between scored replicates stored in the slot assayData of object. The summary is performed as follows:
每个探针的一个单值的计算方法之间的刻痕总结存储插槽assayDataobject复制。摘要如下:
If summary="mean", the average of replicate values is considered;
如果summary="mean",被认为是重复值的平均值;
If summary="median", the median of replicate values is considered;
如果summary="median",被认为是重复值的中位数;
If summary="max", the maximum of replicate intensities is taken;
如果summary="max",复制强度最大;
If summary="min", the minimum is considered, instead;
如果summary="min",被认为是最低的,而不是;
If summary="rms", the square root of the mean squared value of the replicates (root mean square) is taken as a summary function;
如果summary="rms",平均的重复平方值(均方根)的平方根作为汇总函数;
If summary="closestToZero", the value closest to zero is taken as a summary (useful when both sides of the distribution of z-score values are of interest);
如果summary="closestToZero",最接近零值摘要(Z-得分值的分布时双方的利益是有益的);
If summary="furthestFromZero", the value furthest from zero is taken as a summary (useful when both sides of the distribution of z-score values are of interest).
如果summary="furthestFromZero",最远的从零值被视为摘要(Z-得分值的分布时双方的利益是有益的)。
值----------Value----------
The cellHTS object with the summarized scored values stored in slot assayData. This is an object of class assayData corresponding to a single matrix of dimensions Features x 1. Moreover, the processing status of the cellHTS object is updated
cellHTS汇总刻痕值存储在插槽assayData对象。这是一个类assayData到一个单一尺寸特点×1矩阵对应的对象。此外,处理cellHTS对象的状态更新
作者(S)----------Author(s)----------
W. Huber <a href="mailto:huber@ebi.ac.uk">huber@ebi.ac.uk</a>, Ligia Bras <a href="mailto:ligia@ebi.ac.uk">ligia@ebi.ac.uk</a>
参考文献----------References----------
参见----------See Also----------
normalizePlates, summarizeChannels, scoreReplicates, imageScreen.
normalizePlates,summarizeChannels,scoreReplicates,imageScreen。
举例----------Examples----------
data(KcViabSmall)
# normalize[标准化]
x <- normalizePlates(KcViabSmall, scale="multiplicative", method="median", varianceAdjust="none")
# score the replicates[得分复制]
x <- scoreReplicates(x, sign="-", method="zscore")
# summarize the replicates (conservative approach: take the minimum value between replicates)[总结的复制(保守的做法:取最低值之间的复制)]
x <- summarizeReplicates(x, summary="min")
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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