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R语言 cellHTS2包 readHTAnalystData()函数中文帮助文档(中英文对照)

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发表于 2012-2-25 14:17:50 | 显示全部楼层 |阅读模式
readHTAnalystData(cellHTS2)
readHTAnalystData()所属R语言包:cellHTS2

                                        Read a set of plate results obtained from a HTanalyser plate reader
                                         阅读板的结果集,从HTanalyser酶标仪

                                         译者:生物统计家园网 机器人LoveR

描述----------Description----------

Reads input files (specified by the argument filenames) containing replicate data for the same set of assay plates and obtained using an HTanalyser.   The number of replicates corresponds to the number of given files (length(filenames)), while the total number of plates should be given by the argument nrPlates.
读取输入文件(由参数filenames指定)复制使用HTanalyser的数据为同一组的试验板,并获得。复制的数量对应到指定的文件的数量(length(filenames)),而板块的总人数应给出参数nrPlates。


用法----------Usage----------


readHTAnalystData(filenames, path=dirname(filenames), name, nrPlates, verbose=interactive())



参数----------Arguments----------

参数:filenames
vector of characters giving the name(s) of the input file(s) obtained in a HTanalyser plate reader and containing the set of measurements for each set of replicate plates (see details).
矢量字符,输入文件名(S)(S)在HTanalyser酶标仪获得每套重复板包含一套测量(见详情)。


参数:path
can be either a character vector with the same length as filenames or a character of length 1 indicating the path in which to find the input file(s) filenames. By default,  it can extract the path from filenames.
可以是与filenames或一个字符的长度1表示的路径,在其中输入文件()filenames的长度相同的特征向量。默认情况下,它可以从filenames提取的路径。


参数:name
a character of length 1 with the experiment name.
1,实验名称长度的字符。


参数:nrPlates
an integer value indicating the number of plates in the input file(s).
一个整数值,指示板中输入文件的数量(S)。


参数:verbose
a logical value, if TRUE, the function reports some of its intermediate progress. Defaults to the state of interactive().
一个逻辑值,如果属实,功能及其中间体的进展报告。默认的状态interactive()。


Details

详情----------Details----------

This function reads input files obtained in a HTanalyser plate reader for a multi-plate format screening experiment. Data for the same set of replicate measurements of all plates are expected to come in a single input file. So, the argument filenames should be a character vector specifying the name of the input files for each replicate. Each of these files is expected to contain data for a total of nrPlates assay plates. It contains meta-experimental data together with plate measurements in a matrix-like format. The same type of format is expected for each of the nrPlates contained in each input file indicated in filenames. The input files should be suitable to be used as input for readLines.
此功能读取HTanalyser板读者一个多盘格式筛选实验中获得的输入文件。所有板块的重复测量同一组数据,预计将在一个单一的输入文件。因此,参数filenames指定每个重复输入文件的名称应该是一个特征向量。这些文件中的每一个预计包含nrPlates检测板的数据。它包含元的实验数据,连同板测量矩阵状的格式。同类型的格式预计每个nrPlatesfilenames表示每个输入文件中。输入文件应该是合适的输入作为readLines的使用。

The following metadata fields are expected to be repeated along the input file(s) for each assay plate:
预计将沿每个检测板(输入文件)重复下列元数据字段:

Microplate format, indicates the plate format used in the screen. Should be the same across plates.
微孔板格式,显示板在屏幕上使用的格式。整个板块应该是相同的。

Barcode, this field is used to uniquely identify each assay plate.
条码,此字段用来唯一标识每个检测板。

Method ID, indicates the method used to perform the assay. Should be the same for every plate.
方法ID,表示用来执行检测的方法。应该是每板相同。

Data, indicates the type of data and should be the same for every plate.
数据,显示的数据类型,应该是每盘相同。

Units, indicates the units of the readings. Should be the same for every plate.
单位,表示读数的单位。应该是每板相同。

Display format, indicating the numeric format used to display the data measurements. Should be the same along plates.
显示格式,数字格式,用于显示数据的测量。应沿板相同。

This function expects that the next line after the meta field "Display format" for a given plate contains the column numbers (1:ncol) which are then followed by the matrix of measurements in each well. Each entry of the data matrix corresponds to a position in the assay plate with coordinates (row, col), except the first column, which gives the row ID, as upper letters (A, B, ...).
此功能预计元场“显示格式”对于一个给定板后的下一行包含列数(1:ncol),然后由每口井的测量矩阵。数据矩阵中的每个条目对应位置上的字母(A,B ...),从而使该行ID,除第一列在坐标(row, col),检测板。


值----------Value----------

An object of class cellHTS, which extends the class NChannelSet.   After calling this function, the content of the following slots is as follows:
一个对象类cellHTS,延伸类的NChannelSet。调用这个函数后,下面的插槽内容如下:


参数:assayData
an object of class AssayData containing the imported measurement data. Each matrix represents a single channel, and each sample (replicate) corresponds to a column. Thus, the total number of rows in each matrix corresponds to the product between the number of wells per plate and the number of assay plates.  
一个类的对象AssayData包含进口的测量数据。每个矩阵代表一个通道,每个样品(复制),对应一列。因此,每个矩阵中的行的总数相当于每盘的井数和数量的检测板之间的产品。


参数:phenoData
the argument name is stored in its column assay.  
参数name存储在其列assay。


参数:featureData
the information about the plate and well identifiers for each plate measurement are stored in columns plate and well of this slot.  
在列中存储的有关信息板,每块板测量以及标识符plate和well这个槽。


参数:intensityFiles
a list, where each component contains a copy of the measurement data of a given plate, replicate and channel. Its length corresponds to the  number of rows of plateList.
一个列表,其中每个组件包含一个给定的板,复制和通道的测量数据的副本。它的长度相当于plateList行的数目。


参数:plateList
a data.frame containing the columns "Filename", "Plate", "Replicate", "Channel" and "status". Each row of this slot makes the correspondence between a given component (name given in column "Filename") in the list stored in slot intensityFiles (i.e. plate) and its respective plate, replicate and channel number. Thus, this data.frame contains as many rows as the product between the total number of plates, replicates and channels. The last column named status is of type character and contains the string "OK" indicated the success status of the data import.  
数据框,其中包含列“名”,“盘子”,“复制”,“通道”和“状态”。这个槽的每一行作出插槽中存储的名单(“文件名”列中的名称)在一个给定的组件之间的通信intensityFiles(即板)和其各自的板块,复制和通道数量。因此,这个数据框包含尽可能多的行总数板,复制和渠道之间的产品。最后一列名为status的是类型的字符,并包含字符串“OK”表示成功状态数据导入。


作者(S)----------Author(s)----------


W. Huber <a href="mailto:huber@ebi.ac.uk">huber@ebi.ac.uk</a>, Ligia Bras <a href="mailto:ligia@ebi.ac.uk">ligia@ebi.ac.uk</a>



参考文献----------References----------

screens, Genome Biology 7, R66.

参见----------See Also----------

To read a collection of plate result files with measurements of a single plate and replicate, see readPlateList.
阅读与测量一个单一的板一板的结果文件的收集和复制,看到readPlateList。


举例----------Examples----------


    datadir <- system.file("KcCellTiter", package = "cellHTS2")
    x <- readHTAnalystData(filenames = c("KcCellTiter0607.txt", "KcCellTiter0707.txt"), name="KcData", path=datadir, nrPlates=3L)
    x <- configure(x, confFile = "Plateconf.txt", logFile="Screenlog.txt", descripFile = "Description.txt",
           path = datadir)

转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。


注:
注1:为了方便大家学习,本文档为生物统计家园网机器人LoveR翻译而成,仅供个人R语言学习参考使用,生物统计家园保留版权。
注2:由于是机器人自动翻译,难免有不准确之处,使用时仔细对照中、英文内容进行反复理解,可以帮助R语言的学习。
注3:如遇到不准确之处,请在本贴的后面进行回帖,我们会逐渐进行修订。
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