readIntensities(ShortRead)
readIntensities()所属R语言包:ShortRead
Read Illumina image intensity files
阅读Illumina的图像强度文件
译者:生物统计家园网 机器人LoveR
描述----------Description----------
readIntensities reads image "intensity" files (such as Illumina's _int.txt and (optionally) _nse.txt) into a single object.
readIntensities读取图像的强度“的文件(如Illumina的_int.txt”(可选)_nse.txt)到一个单一的对象。
用法----------Usage----------
readIntensities(dirPath, pattern=character(0), ...)
参数----------Arguments----------
参数:dirPath
Directory path or other object (e.g., SolexaPath) for which methods are defined.
目录路径,或其他对象(例如,SolexaPath)方法被定义。
参数:pattern
A length 1 character vector representing a regular expression to be combined with dirPath, as described below, to match files to be summarized.
一个长度为1的特征向量代表一个正则表达式要结合dirPath,如下所述,以匹配要总结的文件。
参数:...
Additional arguments used by methods.
方法使用额外的参数。
Details
详情----------Details----------
Additional methods are defined on specific classes, see, e.g., SolexaPath.
另一种方法是在特定的类的定义,例如,见SolexaPath。
The readIntensities,character-method contains an argument type that determines how intensities are parsed. Use the type argument to readIntensities,character-method, as described below. All readIntensities,character methods accepts the folling arguments:
readIntensities,character-method包含一个参数type,决定如何解析强度。使用typereadIntensities,character-method参数,如下所述。所有的readIntensities,character方法接受folling参数:
withVariability: Include estimates of variability (i.e., from
withVariability:包括变异的估计(即从
verbose: Report on progress when starting to read each file.
详细:启动时读取每个文件的进展情况的报告。
The supported types and their signatures are:
支持的类型和他们的签名是:
Intensities are read from Illumina _cif.txt and _cnf.txt-style files. The signature for this method is
强度从Illumina的_cif.txt和_cnf.txt风格的文件读取。此方法的签名
dirPath, pattern=character(0), ..., type="RtaIntensity", lane=integer(0), cycles=integer(0), cycleIteration=1L, tiles=integer(0), laneName=sprintf("L cycleNames=sprintf("C tileNames=sprintf("s_ posNames=sprintf("s_ withVariability=TRUE, verbose=FALSE
dirPath, pattern=character(0), ..., type="RtaIntensity", lane=integer(0), cycles=integer(0), cycleIteration=1L, tiles=integer(0), laneName=sprintf("L cycleNames=sprintf("C tileNames=sprintf("s_ posNames=sprintf("s_ withVariability=TRUE, verbose=FALSE
lane: integer(1) identifying the lane in which
车道:integer(1)识别车道
cycles: integer() enumerating cycles to be
循环:integer()列举周期
cycleIteration: integer(1) identifying the
cycleIteration:integer(1)确定
tiles: integer() enumerating tile numbers to be
瓷砖:integer()枚举瓦数是
posNames:</dt> character() vectors identifying the lane and cycle directories, and the "pos" and tile file names (excluding the ".cif" or ".cnf" extension) to be
posNames:</ DT>character()向量识别车道和循环目录,和“POS”和瓷砖的文件名(不包括“。到岸价格或。CNF的推广)
The dirPath and pattern arguments are combined as list.files(dirPath, pattern), and must identify a single directory. Most uses of this function will focus on a single tile (specified with, e.g., tiles=1L); the laneName, cycleNames, tileNames, and posNames parameters are designed to work with the default Illumina pipeline and do not normally need to be specified.
dirPath和pattern参数相结合,为list.files(dirPath, pattern),必须确定一个单一的目录。此功能的用途将集中在一个单一的瓷砖(指定);tiles=1L,laneName,cycleNames,tileNames参数,如posNames设计工作与默认Illumina的管道,通常不需要被指定。
Intensities are read from Solexa _pos.txt, _int.txt.p, _nse.txt.p-style file triplets. The signature for this method is
读取从公司Solexa_pos.txt,_int.txt.p,_nse.txt.p风格的文件三胞胎强度。此方法的签名
dirPath, pattern=character(0), ..., type="IparIntensity", intExtension="_int.txt.p.gz", nseExtension="_nse.txt.p.gz", posExtension="_pos.txt", withVariability=TRUE, verbose=FALSE
dirPath, pattern=character(0), ..., type="IparIntensity", intExtension="_int.txt.p.gz", nseExtension="_nse.txt.p.gz", posExtension="_pos.txt", withVariability=TRUE, verbose=FALSE
Files to be parsed are determined as, e.g., paste(pattern, intExtension, sep="").
要解析的文件,例如paste(pattern, intExtension, sep="")决定。
Intensities are read from Solexa _int.txt and _nse.txt-style files. The signature for this method is
公司Solexa_int.txt和_nse.txt风格的文件是只读强度。此方法的签名
dirPath, pattern=character(0), ..., type="SolexaIntensity", intExtension="_int.txt", nseExtension="_nse.txt", withVariability=TRUE, verbose=FALSE
dirPath, pattern=character(0), ..., type="SolexaIntensity", intExtension="_int.txt", nseExtension="_nse.txt", withVariability=TRUE, verbose=FALSE
Files to be parsed are determined as, e.g., paste(pattern, intExtension, sep="").
要解析的文件,例如paste(pattern, intExtension, sep="")决定。
值----------Value----------
An object derived from class Intensity.
来自一个对象类Intensity。
作者(S)----------Author(s)----------
Martin Morgan <mtmorgan@fhcrc.org>,
Michael Muratet <mmuratet@hudsonalpha.org> (RTA).
举例----------Examples----------
fl <- system.file("extdata", package="ShortRead")
sp <- SolexaPath(fl)
int <- readIntensities(sp)
int
intensity(int)[1,,] # one read[一个读]
intensity(int)[[1:2,,]] # two reads, as 'array'[两次读取,如“阵列”]
head(rowMeans(intensity(int))) # treated as 'array'[被视为“阵列”]
head(pData(readInfo(int)))
## Not run: ## RTA Lane 2, cycles 1:80, cycle iteration 1, tile 3[#运行:#退火巷2,周期1:80,循环迭代1,3瓦]
int <- readIntensities("Data/Intensities", type="RtaIntensity",
lane=2, cycles=1:80, tiles=3)
## End(Not run)[#结束(不运行)]
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注:
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