ChromaBlocks(Repitools)
ChromaBlocks()所属R语言包:Repitools
A function to find areas of enrichment in sequencing data
函数找到富集区的测序数据
译者:生物统计家园网 机器人LoveR
描述----------Description----------
This function discovers regions of enrichment in ChIP-seq data, using the method described in Hawkins RD. et al 2010 Cell Stem Cell.
发现此功能在芯片SEQ数据富集的区域,使用中霍金斯RD描述的方法。等2010单元干单元。
用法----------Usage----------
## S4 method for signature 'GRangesList,GRangesList'
ChromaBlocks(rs.ip, rs.input, organism, chrs, ipWidth=100, inputWidth=500, preset=NULL, blockWidth=NULL, minBlocks=NULL, extend=NULL, cutoff=NULL, FDR=0.01, nPermutations=5, nCutoffs=20, cutoffQuantile=0.98, verbose=TRUE, seq.len=NULL)
参数----------Arguments----------
参数:rs.ip
A GRangesList object containing reads from the Immunoprecipited sample. If multiple lanes are supplied, they are pooled.
一个GRangesList对象,其中包含读取从Immunoprecipited样本。如果提供了多个通道,他们汇集。
参数:rs.input
A GRangesList object containing reads from the Input (unenriched) sample. If multiple lanes are supplied, they are pooled.
一个GRangesList对象,它包含的内容从输入(unenriched)的样品。如果提供了多个通道,他们汇集。
参数:organism
The BSgenome object
BSgenome对象
参数:chrs
An character or integer vector with the indicies of the chromosomes of the organism object to analyse
character或integer vectororganism对象的染色体序号为分析
参数:ipWidth
Size in basepairs of the windows to use for the IP samples
碱基大小的窗口,使用的IP样本
参数:inputWidth
Size in basepairs of the windows to use for the Input samples
碱基大小的窗口,使用的输入样本
参数:preset
Either "small", "large" to use cutoffs described in Hawkins et al or NULL (where blockWidth, minBlocks must be specified)
无论是“小”,“大”霍金斯等人或NULL(其中blockWidth,minBlocks必须指定使用)所述截止
参数:blockWidth
Number of adjacent blocks to consider at once
相邻块的数量来考虑一次
参数:minBlocks
The minimum number of blocks required above cutoff
块以上cutoff所需的最低数量
参数:extend
Optional: whether to extend significant blocks until adjacent blocks are less than this value
可选:是否延长显着的块,直到相邻块小于这个值
参数:cutoff
Optional: the cutoff to use to call regions. If left as NULL a cutoff will be chosen which satisfied the specified FDR
可选:截止到使用调用区域。如果离开了NULL将选择一个截止满意指定FDR的
参数:FDR
The target False Discovery Rate; If cutoff is not supplied, one will be chosen to satisfy this value
cutoff如果不提供虚假的发现率目标;,一会被选择,以满足此值
参数:nPermutations
The number of permutations of the data to determine the cutoff at the supplied FDR
数据的排列数,以确定在所提供的cutoff FDR
参数:nCutoffs
The number of different cutoffs to try to satisfy the FDR, a higher value will give finer resolution but longer processing time
编号,以尽量满足不同截止FDR,更高的价值,将给予更高的分辨率,但较长时间处理
参数:cutoffQuantile
The quantile of the RPKM to use as the maximum cutoff tried; a higher value will give lower resolution but may be needed if a cutoff satisfying the FDR cannot be determined with the default value
位数的RPKM使用的最大截止尝试更高的价值,会给分辨率较低,但可能需要如果cutoff的满足FDR的不能用默认值确定
参数:verbose
logical, whether to output commments of the processing
逻辑,是否输出的处理commments的,
参数:seq.len
If sequencing reads need to be extended, the fragment size to be used
如果测序读取需要延长的,要使用片段大小
值----------Value----------
A ChromaResults object.
一个ChromaResults对象。
作者(S)----------Author(s)----------
Aaron Statham
参见----------See Also----------
ChromaResults
ChromaResults
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
注1:为了方便大家学习,本文档为生物统计家园网机器人LoveR翻译而成,仅供个人R语言学习参考使用,生物统计家园保留版权。
注2:由于是机器人自动翻译,难免有不准确之处,使用时仔细对照中、英文内容进行反复理解,可以帮助R语言的学习。
注3:如遇到不准确之处,请在本贴的后面进行回帖,我们会逐渐进行修订。
|