This is a graphical interface to the midas program for detection of alternative splicing detection
这是一个图形界面的替代剪接检测检测到MIDAS程序
用法----------Usage----------
AptMidas()
注意----------Note----------
For more information see Affymetrix Alternative Transcript Analysis Methods for Exon Arrays whitepaper. Before using MiDAS is strongly recomanded to filter out gene level probe sets with low intensity values to avoid searching alternative splicing for probe sets which are not expressed. This can be done using filtering method implemented in oneChannelGUI which define a background intensity threshold on the basis of the intron exon signals of a set of housekeeping genes present in the exon arrays. However it is also possible to use a filter based on the dabg p-value calculated using Affymetrix APT tools. This function will also calculate Splice Index
有关详细信息,请参阅Affymetrix的外显子阵列白皮书替代谈话分析方法。在使用MIDAS强烈recomanded筛选出基因水平探针集低强度值,以避免寻找探针集,这是不是表示剪接。这可以使用过滤方法在oneChannelGUI实施,其中内含子外显子的一套看家基因外显子阵列信号的基础上定义的背景强度阈值。但是它也有可能使用一个过滤器基础上使用Affymetrix APT的工具计算dabg p值。此功能也将接续指数计算