sw(OLIN)
sw()所属R语言包：OLIN
cDNA microarray data of SW480/SW620 experiment
SW480/SW620实验的cDNA微阵列数据
译者：生物统计家园网 机器人LoveR
描述----------Description----------
Gene expression in two cancer cell lines, SW480 and SW620, is compared. The SW480 cell line was derived from a colon tumour of a 50-year old male patient. The second cell line (SW620) originated from a lymph node metastasis of the same patient. Sharing the same genetic background, these cell lines serve as an model of cancer progression.
两种肿瘤单元株SW480和SW620中的基因表达进行了比较。从一个50岁的男性患者的结肠癌SW480单元衍生。第二个单元株（SW620的）起源于同一病人的淋巴结转移。共享相同的遗传背景，这些单元株作为癌症的发展模式。
Target cDNA from SW480 was labelled with Cy3 whereas cDNA from SW620 was labelled with Cy5 using the amino-allyl labelling method. Both cDNA pools were co-hybridised on glass slides with 8448 spots. The spots consisted of 3986 distinct sequence-verified human cDNA clones (Research Genetics, release GF211) printed in duplicates, 84 spots from non-human cDNA clones and a further 154 control spots. Spots were printed by 4x4 pins. The experiment consisted of four replicated arrays derived from separate labelling reactions. Local background spot intensities were extracted by QuantArray software (version2.1). Analysis showed that replicated spots were highly correlated (average Pearson correlation: 0.94). Since this may interfere with the efficiency testing performed (and to reduce the size of the data set for illustration purpose), the replicated spots were not included here. Experimental details and further analysis can be found in Futschik et al. (2002).
目标SW480单元的cDNA，用Cy3标记，而SW620单元的cDNA，用Cy5标记的氨基烯丙基标记方法。这两种基因池共hybridised的玻片上8448点。景点包括3986序列验证的独特人类cDNA克隆在重复印刷（遗传学研究，释放GF211），84点从非人类的cDNA克隆和154控制点。斑印的4x4针脚。实验分为四个复制的阵列来自单独的标记反应。当地背景当场强度提取QuantArray软件（version2.1）。分析表明，复制点高度相关（Pearson相关系数平均：0.94）。因为这可能会妨碍执行效率测试（作说明用途的数据集，以减少大小），这里不包括复制点。实验的细节，并进一步分析可以发现在Futschik等。 （2002年）。
用法----------Usage----------
data(sw)
格式----------Format----------
An object of class “marrayRaw”
一个对象的类“marrayRaw”
源----------Source----------
The data was produced and provided by Sharon Pattison of the Cancer Genetics lab and Aaron Jeffs of
沙龙派特森癌症遗传学实验室和亚伦杰夫斯的数据生产和提供
参考文献----------References----------
<h3>See Also</h3>
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注：
注1：为了方便大家学习，本文档为生物统计家园网机器人LoveR翻译而成，仅供个人R语言学习参考使用，生物统计家园保留版权。
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发表于 2012-2-26 08:23:48
