mannwhitneyCtData(HTqPCR)
mannwhitneyCtData()所属R语言包:HTqPCR
Differentially expressed features with qPCR: Mann-Whitney
差异表达的特征与定量PCR:采用Mann-Whitney
译者:生物统计家园网 机器人LoveR
描述----------Description----------
Function for calculating p-values across two groups for the features present in high-throughput qPCR data, such as from TaqMan Low Density Arrays. Also known as two sample Wilcoxon test.
跨两个组的功能的P-值计算功能介绍,如高通量qPCR数据的TaqMan低密度阵列。也被称为两样本秩和检验。
用法----------Usage----------
mannwhitneyCtData(q, groups = NULL, calibrator, alternative = "two.sided", paired = FALSE, replicates = TRUE, sort = TRUE, stringent = TRUE, p.adjust = "BH", ...)
参数----------Arguments----------
参数:q
qPCRset object.
qPCRset对象。
参数:groups
factor, assigning each sample to one of two groups.
因素,每个样本分配到两组。
参数:calibrator
which of the two groups is to be considered as the reference and not the test? Defaults to the first group in groups.
两组的是要考虑作为参考,而不是测试?默认为第一组在groups。
参数:alternative
character string (first letter is enough), specifying the alternative hypothesis, "two.sided" (default), "greater" or "less".
字符串(第一个字母是足够的),指定替代假说“,two.sided”(默认),“大”或“少”。
参数:paired
logical, should a paired t-test be used.
逻辑,应使用配对t检验。
参数:replicates
logical, if replicated genes are present on the array, the statistics will be calculated for all the replicates combined, rather than the individual wells.
逻辑,如果复制的基因是存在阵列上,统计计算的重复组合,而不是个别井。
参数:sort
boolean, should the output be sorted by p-values.
布尔值,p值进行排序输出。
参数:stringent
boolean, for flagging results as "Undetermined". See details.
布尔,标记为“未决定用途”的结果。查看详情。
参数:p.adjust
character string, which method to use for p-value adjsutment for multiple testing. See details.
字符串,该方法使用多重检验的p值adjsutment。查看详情。
参数:...
any other arguments will be passed to the wilcox.test function.
任何其他参数将被传递给wilcox.test功能。
Details
详情----------Details----------
Once the Ct values have been normalised, differential expression can be calculated. This function deals with just the simple case, where there are two types of samples to compare. For a parametric test see ttestCtData and limmaCtData for more complex studies.
Ct值已标准化后,可以计算出差异表达。这个函数只是简单的例子,那里有两种类型的样品进行比较处理。为参数测试ttestCtData和limmaCtData更复杂的研究。
The underlying statistics is calculated by wilcox.test. Due to the high possibility of ties for each feature between samples, the test is run with exact=FALSE.
底层的统计数字计算wilcox.test。由于每个样品之间的功能关系的可能性很高,运行测试用exact=FALSE。
All results are assigned to a category, either "OK" or "Undetermined" depending on the input Ct values. If stringent=TRUE any unreliable or undetermined measurements among technical and biological replicates will result in the final result being "Undetermined". For stringent=FALSE the result will be "OK" unless at least half of the Ct values for a given gene are unreliable/undetermined.
所有的结果都分配到一个类别,可以根据输入的Ct值“OK”或“未决定用途”。如果stringent=TRUE之间的技术和生物复制任何不可靠的或未定测量,将导致最终的结果是“未决定用途”。 stringent=FALSE结果将是“确定”,除非至少有一半的一个特定基因的Ct值是不可靠/未定。
The argument p.adjust is passed on to the p.adjust function. Options include e.g. "BH" (Benjamini & Hochberg, the default), "fdr" and "bonferroni". See p.adjust for more information on the individual methods.
传递的参数p.adjustp.adjust函数。选项包括例如“波黑”(Benjamini Hochberg,默认),“FDR”和“邦弗朗尼”。看到p.adjust个别方法的更多信息。
值----------Value----------
A data.frame containing the following information:
数据框包含以下信息:
参数:genes
The names of the features on the card.
卡上的功能的名称。
参数:feature.pos
The featurePos of the genes. If replicated genes are used, the feature positions will be concatenated together.
featurePos的基因。如果使用复制的基因,该功能的职位将被连接在一起。
参数:MB.test
The name and value of the test statistic.
检验统计量的名称和值。
参数:p.value
The corresponding p-value.
相应的p值。
参数:ddCt
The delta delta Ct values.
DeltaDeltaCt值。
参数:FC
The fold change; 2^(-ddCt).
倍; 2 ^(DDCT)。
参数:meanCalibrator
The average expression level of each gene in the calibrator sample(s).
平均每个基因的表达水平在校准样品(S)。
参数:meanTarget
The average expression level of each gene in the target sample(s).
平均每个基因表达水平的目标样本(S)。
参数:categoryCalibrator
The category of the Ct values ("OK", "Undetermine") across the calibrator.
Ct值(“OK”,“Undetermine”)跨校准类别。
参数:categoryTarget
Ditto for the target.
同上,用于目标。
作者(S)----------Author(s)----------
Heidi Dvinge
参见----------See Also----------
wilcox.test, ttestCtData, limmaCtData. plotCtRQ and plotCtSignificance can be used for visualising the results.
wilcox.test,ttestCtData,limmaCtData。 plotCtRQ和plotCtSignificance可以使用可视化的结果。
举例----------Examples----------
# Load example preprocessed data[加载预处理数据的例子]
data(qPCRpros)
# Test between two groups, collapsing replicated features[两组试验,倒塌复制功能]
diff.exp <- mannwhitneyCtData(qPCRpros[,1:4], groups=factor(c("A", "B", "B", "A")), calibrator="B")
diff.exp[1:10,]
# The same test, taking replicated features individually[相同的测试,个别复制功能]
diff.exp <- mannwhitneyCtData(qPCRpros[,1:4], groups=factor(c("A", "B", "B", "A")), calibrator="B", replicates=FALSE)
# Using another method for p-value ajustment[另一种方法,使用p值大庆石油管理局]
diff.exp <- mannwhitneyCtData(qPCRpros[,1:4], groups=factor(c("A", "B", "B", "A")), calibrator="B", p.adjust="holm")
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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