NormiR(ExiMiR)
NormiR()所属R语言包:ExiMiR
miRNA raw data normalization function (high level)
miRNA的原始数据的标准化功能(高层次)
译者:生物统计家园网 机器人LoveR
描述----------Description----------
This function converts an AffyBatch object into an ExpressionSet object performing both normalization and summarization. By default it uses the spike-in probe-based normalization method and the median summarization. In case the spike-in probe-based method cannot be applied, a median normalization is executed instead. Several options allow to force the execution of the spike-in probe-based normalization and to fine-tune the resulting correction functions.
此功能转换成一个ExpressionSet执行标准化和总结的对象AffyBatch对象。默认情况下,它使用的尖峰探针为基础的归一化法和中位数的总结。穗的情况下探针为基础的方法不能套用,中位数标准化执行。几个选项允许强制执行穗探针为基础的标准化和微调校正功能。
用法----------Usage----------
NormiR( abatch,
method=c("spikein","mean","median"),
background.correct=FALSE,
verbose=TRUE,
figures.show=TRUE,
figures.output=c("display","file"),
out.type=c("ExpressionSet", "data.frame"),
min.corr=0.5,
loess.span=-1,
extrap.points=2,
extrap.method=c("mean","linear"),
force.zero=FALSE,
cover.ext=0.5,
cover.int=1/3,
max.log2span=1)
参数----------Arguments----------
参数:abatch
AffyBatch object
AffyBatch对象
参数:method
Character vector. By default, spikein method is used. mean or median can also be selected and are used in case the 'spike-in' method can not be applied.
特征向量。默认情况下,spikein方法。 mean或median也可以被选中,并使用情况下,“穗”的方法不能适用。
参数:background.correct
Logical. Default value is FALSE. If TRUE, the rma background correction is applied.
逻辑。默认值是FALSE。如果TRUE,rma背景校正适用于。
参数:verbose
Logical. Default value is TRUE; some details are provided on the console
逻辑。默认值为TRUE;一些细节上提供的控制台
参数:figures.show
Logical. Default value is TRUE. Control figures are generated for the spikein method.
逻辑。默认值是TRUE。数字控制产生spikein方法。
参数:figures.output
Character vector. By default, display is used. Figures are shown to the screen. Using file generates the figures in PDF format in the working directory.
特征向量。默认情况下,display使用。数字显示在屏幕上。使用file在工作目录中的PDF格式生成的数字。
参数:out.type
Character vector. Default value is ExpressionSet. The object type output by NormiR.
特征向量。默认值是ExpressionSet。由NormiR的对象类型的输出。
参数:min.corr
Numeric. Default value is 0.5. Minimal allowed value for the average of the off-diagonal elements of the Pearson correlation matrix of the spike-in probeset intensities across the arrays.
数字。默认值是0.5。关闭整个阵列穗在probeset强度的Pearson相关矩阵对角线元素的平均最小允许值。
参数:loess.span
Numeric. Default value is -1, which corresponds to a loess smoothing neighbourhood spanning a fraction 3/(number of spike-in probesets) of the total number of points. Other positive values are allowed, see the span argument of the R loess function
数字。默认值是-1,这相当于一个黄土平滑居委会跨越点总数的3 /(穗在probesets号)的一小部分。其他正面的价值观是允许的,看到的span的Rloess函数参数
参数:extrap.points
Numeric. Default value is 2. The number of spike-in probesets used in the high-intensity extrapolation of the normalization correction function.
数字。默认值是2。穗的数量在probesets用在高强度的标准化校正功能推断。
参数:extrap.method
Character vector. Default value is mean. The method used for the high-intensity extrapolation of the normalization correction function.
特征向量。默认值是mean。该方法用于标准化校正功能的高强度的推断。
参数:force.zero
Logical. Default value is FALSE. If TRUE, it forces the normalization correction functions to have zero values at the lower end of the probe intensity range.
逻辑。默认值是FALSE。如果TRUE,它迫使标准化校正功能,在探针强度范围的低端值为零。
参数:cover.ext
Numeric. Default value is 1/2. Minimal allowed relative coverage of the spike-in probesets intensities. It is computed as the ratio between the intensity range covered by the spike-in probes and the one covered by all probes on the array.
数字。默认值是1/2。最小允许相对覆盖的在穗probesets强度。据计算比之间的强度范围涵盖穗探针和一个阵列上的所有探针涵盖。
参数:cover.int
Numeric. Default value is 1/3. Maximal allowed relative intensity interval between two consecutive spike-in probesets. It is computed as the largest intensity difference between two consecutive spike-in probesets divided by the overall probe intensity range.
数字。默认值是1/3。最大允许连续两穗在probesets的相对强度间隔。据计算整体探针强度范围分为两个连续尖峰IN probesets最大的强度差。
参数:max.log2span
Numeric. Default value is 1. Gives the maximal (log2) intensity interval allowed for the probes belonging to one spike-in probeset.
数字。默认值是1。给人最大的(log2)强度属于穗在probeset探针允许的时间间隔。
Details
详情----------Details----------
See accompanying vignette.
请参阅随附的小插曲。
值----------Value----------
An ExpressionSet object or a data.frame object, depending on the out.type option
ExpressionSet对象或data.frame对象,根据out.type选项
作者(S)----------Author(s)----------
Sylvain Gubian, Alain Sewer, PMP SA
举例----------Examples----------
data(galenv)
data(GSE20122)
eset.spike <- NormiR(GSE20122)
eset.spike
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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