betweenLaneNormalization-methods(EDASeq)
betweenLaneNormalization-methods()所属R语言包:EDASeq
Methods for Function betweenLaneNormalization in Package EDASeq
为函数在包装EDASeq betweenLaneNormalization方法
译者:生物统计家园网 机器人LoveR
描述----------Description----------
Between-lane normalization for sequencing depth and possibly other distributional differences between lanes.
测序深度和其他可能的分布差异之间的车道,车道之间的标准化。
用法----------Usage----------
betweenLaneNormalization(x, which=c("median","upper","full"), offset=FALSE)
参数----------Arguments----------
参数:x
A numeric matrix representing the counts or a SeqExpressionSet object.
一个数字矩阵代表计数或SeqExpressionSet对象。
参数:which
Method used to normalized. See the details section and the reference below for details.
使用的方法来归。看到细节部分和参考下面的细节。
参数:offset
Should the normalized value be returned as an offset leaving the original counts unchanged?
应该归一化值返回一个偏移离开原来的数量不变?
Details
详情----------Details----------
This method implements three normalizations described in Bullard et al. (2010). The methods are:
这种方法实现了布拉德等三个归。 (2010年)。其方法是:
median: a scaling normalization that forces the median of each lane to be the same.
median:一个比例标准化,迫使每个车道的中位数是相同的。
upper: the same but with the upper quartile.
upper:相同的,但与上四分位数。
full: a non linear full quantile normalization, in the spirit of the one used in microarrays.
full:非线性充分位数标准化,在芯片中使用的精神。
方法----------Methods----------
It returns a matrix with the normalized counts if offset=FALSE or with the offset if offset=TRUE.
它返回矩阵如果offset=FALSE如果offset=TRUE或偏移归计数。
It returns a linkS4class{SeqExpressionSet} with the normalized counts in the exprs slot if offset=FALSE or with the offset in the offset slot and the original counts in the exprs slot if offset=TRUE.
它返回一个linkS4class{SeqExpressionSet}插槽归计数exprs如果offset=FALSE或offsetexprs插槽插槽和原始计数抵消如果offset=TRUE。
作者(S)----------Author(s)----------
Davide Risso.
参考文献----------References----------
举例----------Examples----------
library(yeastRNASeq)
data(geneLevelData)
data(yeastGC)
sub <- intersect(rownames(geneLevelData),names(yeastGC))
mat <- as.matrix(geneLevelData[sub,])
data <- newSeqExpressionSet(mat,phenoData=AnnotatedDataFrame(data.frame(conditions=factor(c("mut","mut","wt","wt")),row.names=colnames(geneLevelData))),featureData=AnnotatedDataFrame(data.frame(gc=yeastGC[sub])))
norm <- betweenLaneNormalization(data,which="full",offset=FALSE)
转载请注明:出自 生物统计家园网(http://www.biostatistic.net)。
注:
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